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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and <t>pLX304</t> (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. <t>The</t> <t>pLenti-puro</t> (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. <t>The</t> <t>pLenti-puro</t> (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
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plx304 lentiviral vector  (Addgene inc)
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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. <t>The</t> <t>pLenti-puro</t> (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. <t>The</t> <t>pLenti-puro</t> (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.
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A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

Journal: bioRxiv

Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

doi: 10.64898/2026.01.23.701386

Figure Lengend Snippet: A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

Techniques:

A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

Journal: bioRxiv

Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

doi: 10.64898/2026.01.23.701386

Figure Lengend Snippet: A) the overall scheme for using PCR tagged cells in a mixed xenograft is shown. The pLenti-puro (B) and pLX304 (C) based sets of lentiviral tagging vectors are shown schematically, with the expanded regions above indicating the Tags and the locations of primer pairs.

Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

Techniques:

qPCR analysis of six pools of HCT116 cells, each with one of the six pLenti-puro based Tag vectors (Tag in cells: 1-6, shades of green). Following DNA isolation and pre-amplification, each sample was analyzed by qPCR for all six Tags as well as with the internal primer pair. Data are plotted (grouped by Tag primer pair used for qPCR) as the delta Ct (internal minus Tag amplicon).

Journal: bioRxiv

Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors

doi: 10.64898/2026.01.23.701386

Figure Lengend Snippet: qPCR analysis of six pools of HCT116 cells, each with one of the six pLenti-puro based Tag vectors (Tag in cells: 1-6, shades of green). Following DNA isolation and pre-amplification, each sample was analyzed by qPCR for all six Tags as well as with the internal primer pair. Data are plotted (grouped by Tag primer pair used for qPCR) as the delta Ct (internal minus Tag amplicon).

Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites.

Techniques: DNA Extraction, Amplification